Department of Mechanical and Aerospace EngineeringOUR PEOPLE
Yi-Kuen LEE 李貽昆
PhD, UCLA
Associate Professor of Mechanical and Aerospace Engineering
Associate Director of Institute of Integrated Microsystem
Associate Director of Center for Bioengineering and Biomedical Devices



Graduated from Agricultural Machinery Department (now Bio-Industrial Mechatronics Engineering Department), National Taiwan University (NTU), and then obtained MS degree in Institute of Applied Mechanics, NTU. After completing two-year's military service in Taiwan, he went to the United States and obtained a Ph.D. degree in Mechanical Engineering from the University of California, Los Angeles under the guidance of Dr. Chih-Ming Ho. He is an Associate Professor in the Department of Mechanical Engineering and a Research Affiliate in the Institute of Integrated Microsystem and Bioengineering Graduate Program at HKUST.

Teaching Activities

MECH5950 Introduction to Microsystems: Technology and Devices

 

Research Interests

Bio-Microelectromechanical System (Bio-MEMS) and Lab-on-a-chip: micro biosensors, micro electroporation chips
Nanoelectromechanical System (NEMS)
Micro chaotic mixing: micro spatial-temporal chaotic mixer
MEMS key technology development: ICP Deep RIE process modeling and optimization using Design of Experiments
Micro sensors using nanocomposite materials
Fluorescence video microscopy for single molecule analysis
Micro bubble actuators for integrated microfluidic systems
 

Research Projects

  • HK RGC CERG 2004-2006, Integrated nano bubble actuator (PI).
  • HK RGC CERG 2005-2006, Low-Voltage Micro Pulsed RF Electroporation Cell Chip for DNA Transfer  (PI).
  • HK RGC CERG 2006-2007, Nonlinear Electrophoresis Dynamics of Large DNA Molecules in Micro Electrophoresis Chip with Nanopillar Arrays (PI)
  • HK RGC CERG 2007-2008, Microelectroendocytosis Cell Chips for DNA Transfection with High Cell Viability (PI)
  • KAUST-HKUST 2008-2012, Joint Microfluidics Research Project on micro electroporation (Co-I)
  • HK RGC CERG 2007-2008, The TiO2-modified UV-C Disinfection Process for Inhibition of Post-Irration Bacteria Recovery: Mechanism Assessment and Process Development (Co-I, with Prof Chii Shang, HKUST)
  • HK RGC CERG 2007-2008, An Integrated Microfluidic Chip for Single Cell Electrophysiological Studies within Picoliter Volume (Co-I, with Prof Mo Yang, HK PolyU).
  • HKUST Emerging High Impact Areas (EHIA) Grant 2005/06, Integrated Smart Biometrics Systems (Co-I, with Prof Amine Bermak).
  • HKUST Emerging High Impact Areas (EHIA) Grant 2005/06, Novel DNA-Aptamer based Bioanalytical Microsystem (Co-I, with Prof I-Ming Hsing)
  • USA Natl Sci. Foundation Intl. Grant 2003~2007, Microfluidic Mixers Using Nanoengineered Responsive Polymer Surfaces (PI, with Prof Qiao Lin, Carnegie Mellon Univ., USA).
  • HKUST PDF Matching Fund, 2005-2006, Development of technology for integrated nano bubble actuator devices (PI).
  • F-HK18/05T 2006/2007  France/Hong Kong Joint Research Scheme,  Integrated Microfluidic System Using Giant Electrorheological Fluid , with  Prof Patrick Tabeling, ESPCI, Paris, France (PI).
  • F-HK07/08.EG07 2007/2008 France/Hong Kong Joint Research Scheme, Giant Electrorheological Fluid Based-Digital Microfluidics, with Prof Patrick Tabeling, ESPCI, Paris, France (PI).

 

Selected Publications

Book Chapters

  • P. Tabeling and Y.K. Lee, “Micro/nanofluidic Processes,” in Micro/Nano Technology Systems for Biomedical Applications: Microfluidics, Optics, and Surface Chemistry, Chapter 2, C.M. Ho (ed.), pp.33,52, Oxford University Press, 2010.
  • P. Cheng, Y.K. Lee and P. Deng, Thermal Bubbles in Encyclopedia of Micro- and Nano-Fluidics, D. Li (ed.), Springer, 2008.

Journal Papers

  • R. Lin, D.C. Chang and Y.-K. Lee, “Single-Cell Electroendocytosis on a Micro Chip Using In Situ Fluorescence Microscopy,” Biomedical Microdevices, 2011 (in press).
  • M. Lee, L.S.L. Cheung, Y.-K. Lee and Y. Zohar, "Design, Fabrication and Characterization of a Thermal Microsystem Integrated with Pressure and Temperature Microsensors," Journal of Micromechanics and Microengineering, 2011 (in review).
  • M. Lee, Y.-K. Lee and Y. Zohar, “Single-phase liquid flow forced convection under nearly uniform heat flux boundary condition in microchannels,” Journal of Micromechanics and Microengineering, 2011 (in review).
  • R. Lin, D.C. Chang and Y.-K. Lee, “Temperature-Dependent Single-Cell Endocytosis on a Micro System,” Lab on a Chip, 2011 (in review).
  • S. Sun, G. Yin, Y.-K. Lee, J.T.Y. Wong, T.-Y. Zhang, “Effects of deformability and thermal motion of lipid membrane on electroporation: By molecular dynamics simulations,” Biochemical and Biophysical Research Communications, Vol. 404, No. 2, 684-688, 2011.
  • S. Wang, K. Liu, J. Liu, Y.-K. Lee, Z T.-F. Yu, E.K. Lee, X. Xu, J. Reiss, L.W.K. Chung, J. Huang, M. Rettig, A. Pantuck, D. Silegson, K.N. Duraiswamy, C.K.-F. Shen and H.-R.  
  • Tseng, “Highly Efficient Capture of Circulating Tumor Cells Using Nanostructured Silicon Substrates with Integrated Chaotic Micromixers,” Angewandte Chemie International  Ed., Vol.50 pp.3084-3088, 2011 (http://dx.doi.org/10.1002/anie.201005853): Selected as the cover page in the issue of 21 Mar 2011.
  • W. Wang, Y.C. Chan and Y.-K. Lee, “Biased Reptation Model with Electroosmosis for DNA Electrophoresis in Microchannels with Sub-Micron Pillar Array,” Journal of Micromechanics and Microengineering, 2011 (http://dx.doi.org/10.1088/0960-1317/21/8/085031).
  • L. Chen, Z. Xiao, P. C. H. Chan, Y.-K. Lee, and Z. Li, "A Comparative Study of Droplet Impact Dynamics on a Dual-scaled Superhydrophobic Surface and Lotus Leaf," Applied Surface Science, 2011.
  • L. Chen, Z. Xiao, P.C.H. Chan, Y.-K. Lee, “Static and Dynamic Characterization of Robust Superhydrophobic Surfaces Built from Nano-flowers on Silicon Micro-Post Arrays,”Journal Micromechanics and Microengineering, Vol. 20. No. 10,  105001 (8pp), 2010 (dx.doi.org/10.1088/0960-1317/20/10/105001).
  • B.J. Xu, Z.B. Liu, J.J. Yu, Y.-K. Lee and M. Yang , A PDMS Microfluidic System with Poly (ethylene glycol)/SU-8 Based Apertures for Planar Whole Cell Patch-Clamp Recordings,Sensors and Actuators A, 2009 (dx.doi.org/10.1016/j.sna.2009.12.010).
  • Y.-Y. Ling, Y. Zhang, W. Wen, P. Tabeling and Y.-K. Lee, “An Integrated Micro Giant Electrorheological Fluid Valves for Micro Flow Cytometry,” Journal of Micro/Nanolithography, MEMS and MOEMS, Vol. 8, No. 2, 021103, 2009.
  • Y.C. Chan, Y. Zohar and Y.-K. Lee, “Effects of Embedded Sub-Micron Pillar Arrays in Microfluidic Channels on Large DNA Electrophoresis,” Electrophoresis, Vol. 30, No. 18, pp.3242-3249, 2009.
  • H. He, D.C. Chang and Y.-K. Lee, “Nonlinear Current Response of Micro Electroporation and Resealing Dynamics for Human Cancer Cells,” Bioelectrochemistry, Vol. 72, No. 2, pp.161-168, 2008.
  • H. He, D. C. Chang and Y.-K. Lee, "Using a Micro Electroporation Chip to Determine the Optimal Physical Parameters in the Uptake of Biomolecules in HeLa Cells," Bioelectrochemistry, Vol. 71, No. 2, pp.363-368, 2007.
  • X. Cao, W.-J. Chiang, Y.-C. King and Y.-K. Lee, "Electromagnetic Energy Harvesting Circuit With Feedforward and Feedback DC-DC PWM BoostConverter for Vibration Power Generator System," IEEE Transactions on Power Electronics, Vol. 22, No. 2, pp. 679-685, 2007.
  • Y.-K. Lee, L.M. Lee, W.L.W. Hau and Y. Zohar., “Two-Dimensional Analysis of Electrokinetically-driven Out-of-plane Vorticies in a Microchannel Liquid Flow Using Patterned Surface Charge,” Journal of Microelectromechanical Systems, Vol. 16, No. 1, pp.58-67, 2007.
  • Y.-K. Lee, C. Shih, P. Tabeling and C.M. Ho, “Experimental Study and Nonlinear Dynamic Analysis of Time-periodic Micro Chaotic Mixers,” Journal of Fluid Mechanics, Vol. 575, pp.425-448, 2007.
  • H. He, D. C. Chang and Y.-K. Lee, "Micro Pulsed Radio-Frequency Electroporation Chip," Bioelectrochemistry, Vol. 68, No.1, pp. 89-97, 2006. Abstract.
  • P. Deng, Y.-K. Lee and P. Cheng, "Two-dimensional Micro Bubble Actuator Array to Enhance the Efficiency of Molecular Beacon Based DNA Micro   Biosensor,"  Biosensors and Bioelectronics, Vol. 21, No. 8, pp. 1443-1450, 2006.   Abstract 
  • L.M. Lee, W. L.W. Hau, Y.-K. Lee, Y. Zohar, "In-plane Vortex Flow in Microchannels Generated by Electroosmosis with Patterned Surface Charge," Journal of Micromechanics and Microengineering, Vol. 16, No. 1, pp.17-26, 2006.  Abstract
  • X. Niu, W. Wen and Y.-K. Lee, “Electrorheological-fluid-based Microvalves,” Applied Physics Letters, Vol. 87, 243501 (1-3), 2005. Abstract
  • P. Deng, Y.-K. Lee and P. Cheng, "An Experimental Study of Heater Size Effect on Micro Bubble Generation,"  Intl. J. Heat and Mass Transfer, Vol. 49, No. 15-16, pp.2535-2544, 2006.
  • Z. Jia, Y.-K. Lee, Q. Fang and C. W. Huie, "Video Imaging of Sample Zone Narrowing and Dispersion in a Glass Microchip: the Effect of Organic Solvent(Acetonitrile)-salt Mixtures in the Sample Matrix and Surfactant Micelles in the Running Buffer," Electrophoresis , Vol. 27, No.5-6, pp.1104-1111, 2006.
  • Y. C. Chan, Y.-K. Lee and Y. Zohar, "High-throughput Design and Fabrication of an Integrated Microsystem with High Aspect-Ratio Sub-Micron Pillar Arrays for Free-Solution Micro Capillary Electrophoresis," Journal of Micromechanics and Microengineering, Vol. 16, No. 4, pp. 699-707, 2006.
  • B. Xu, Y.-K. Lee, Q.H. Jin, J.L. Zhao and C.M. Ho, "Multi-layer SU-8 Microdispenser for Microarray Assay," Sensors and Actuators A: Physical, Vol. 132, No. 2,  pp. 714-725, 2006.

Conference Presentations

  • H. Shagoshtasbi and Y.-K. Lee, “A New Equivalent Circuit Model for Micro Electroporation Systems,” the 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS '11), pp.697-700, Kaohsiung, Taiwan, Feb 20-23, 2011.
  • P. Deng, J. Zhou, G. Li, D.C. Chang and Y.-K. Lee, “DNA Transfection of Bone Marrow Mesenchymal Stem Cells Using Micro Electroporation Chips,” the 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS '11), pp.98-101, Kaohsiung, Taiwan, Feb 20-23, 2011.
  • B. Lu, J.C-H. Lin, Z. Liu, Y.-K. Lee and Y.-C. Tai, “Highly Flexible, Transparent and Patternable Parylene-C Superhydrophobic Films with High and Low Adhesion,” IEEE MEMS 2011, pp.1143-1146, Cancun, Mexico, 23-27 Jan 2011.
  • J. C.-H. Lin, G. Lam, B. Lu, P. Deng, Y.-K. Lee, Y.-C Tai, “Creep of Parylene C-Film,” the 16th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers’11), pp.2698-2701, Beijing, China, Jun 5-9, 2011.
  • R. Lin, D. C. Chang, and Y.-K. Lee, "Study of Temperature Effect on Single-Cell Fluid-Phase Endocytosis Using Micro Cell Chips and Thermoelectric Devices," the 14-th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS'10), pp.962-964, Groningen, Netherlands, 3-7 Oct, 2010.
  • W. Wang and Y.-K. Lee, "Optimum Peclet Numbers for Accurate Measurement of Electroosmotic Mobility of Complex DNA Buffers in Micro/Nanofludics," the 14-th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS'10), pp.1766-1768, Groningen, Netherlands, 3-7 Oct, 2010.
  • R. Lin, D. C. Chang, and Y.-K. Lee, "Temperature Effect on Single-Cell Electroendocytosis Using a Cell-Array Chip with Micro Temperature Sensors and a Peltier Thermoelectric Device," 2010 International Symposium on Microchemistry and Microsystems (ISMM 2010), Hong Kong, pp. 47-48, 28-30 May, 2010.
  • L. Chen and Y.-K. Lee, “New Dimensionless Number for Superhydrophobicity Study of Micron/Submicron Patterned Surfaces,” 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), Hong Kong, pp.1051-1054, 24-28 Jan 2010.
  • P. Deng, G. Yin, T.-Y. Zhang, D.C. Chang and Y.-K. Lee, “Theoretical and Experimental Study of Electroporation of Red Blood Cells Using MEMS Technology,” the 5th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, pp.588-591, 20-23 Jan 2010.
  • X. Jiang, Y. Ling, C. Liu, S. Yang and Y.-K. Lee, “Electrorheological Characterization of Ultrathin Titanium Dioxide Nanorod Solutions Using Microfluidics,” the 5th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, pp.362-365, 20-23 Jan 2010.
  • W. Wang, Y.C. Chan and Y.-K. Lee, “Biased Reptation Model with Electroosmosis for DNA Electrophoresis in Microchannels with Sub-Micron Pillar Array,” the 5th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, pp.425-428, 20-23 Jan 2010.
  • Y. Ling, H. Liu and Y.-K. Lee, “A Micro Flow Cytometry System for Study of Marine Phytoplankton from Costal Waters of Hong Kong,”  the 5th Annual IEEEInternational Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, pp.580-583, 20-23 Jan 2010.
  • G.-Y. Yin, R. Lin, T.-Y. Zhang, D.C. Chang and Y.-K. Lee, “Systematic Study of Electroporation of Mouse Myoblast Cells on Microchips,”  the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Jeju, Korea, pp.509-511, 1-5 Nov 2009.
  • R. Lin, D.C. Chang and Y.-K. Lee, “Study of Single-Cell Electroendocytosis Using Bio-MEMS Technology,” 15th International Conf. on Solid-State Sensors, Actuators and Microsystems (Transducers 2009), Denver, CO. USA, pp.2366-2369, 21-25 Jun 2009.
  • L. Chen, Z. Xiao, P.C.H. Chan and Y.-K. Lee, “Dual-Scaled Superhydrophobic Nano-Flower Surfaces,” 15th International Conf. on Solid-State Sensors, Actuators and Microsystems (Transducers 2009), Denver, CO, USA, pp.441-444, 21-25 Jun 2009.
  • B.J. Xu, Z.B. Liu, J.J. Yu, Y.-K. Lee and M. Yang , “A PDMS Planar Patch-Clamp Array Chip with Poly (Ethylene Glycol)/SU-8 Based Cell-Patch Interface, IEEE MEMS 2009, Sorrento, Italy, pp.395-398, 25-29 Jan 2009.
  • H. He, D.C. Chang and Y.-K. Lee, “Battery-Powered Miniaturized Electroporation System for In-Situ Gene Transfer in Stem Cell Research, IEEE NEMS 2009, pp. 458-462, Shenzhen, China, Jan 5-8, 2009.
  • K.-Y. Fong, R. Lin, P. Tabeling and Y.-K. Lee, “A Droplet-Based Real-Time PCR Microchip with Self-Sening Heaters for Bacterial Detection,” IEEE NEMS 2009, pp.143-146,Shenzhen, China, Jan 5-8, 2009.
  • Y.Y. Ling, Y. Zhang, W. Wen, P. Tabeling and Y.-K.  Lee, “Design and Fabrication of Integrated Micro GER Valves for Micro Flow Cytometry,” Asia-Pacific Conference on Transducers and Micro-Nanotechnology (APCOT 2008), Tainan, Taiwan, 22-25 Jun 2008.
  • J. Lu, M. Lu, A. Bermak and Y.-K. Lee, “Study of Piezoresistance Effect of Carbon Nanotube-PDMS Composite Materials for Nanosensors,” IEEE Nano 2007, Hong Kong, pp.1240-1243, Aug 2-5, 2007.
  • M. Lu, A. Bermak, Y.-K. Lee, "Fabrication Technology of Piezoresistive Conductive PDMS for Micro Fingerprint Sensors," IEEE MEMS 2007, Kobe, Japan, pp.251-254, Jan 21-25,  2007.
  • Y.C. Chan, Y.-K. Lee and Y. Zohar, "Pillar Size Effect on DNA Electrophoresis in Microchip with Sub-micron Pillar Arrays," IEEE MEMS 2007, Kobe, Japan, pp.413-416, Jan 21-25, 2007.
  • M. Lee, Y.-K. Lee and Y. Zohar, "Two-Phase Flow Oscillations in Microchannel Convective Boiling," IEEE MEMS 2007, Kobe, Japan, pp.635-638, Jan 21-25, 2007.
  • H. He, D.C. Chang and Y.-K. Lee, "Nonlinear Transmembrane Current Response of Micro Electroporation for Human Cancer Cells," IEEE NEMS 2007, Bangkok,Thailand, pp.258-262, Jan 16-19, 2007.
  • M. Lee, Y.-K. Lee and Y. Zohar, "The Nusselt Number in Single-Phase Liquid Flow Forced Convection in Microchannels ," IEEE NEMS 2007, Bangkok, Thailand, pp.502-506, Jan 16-19, 2007.
  • H. He, Y.-K. Lee and D.C. Chang, "Molecular Size Effect on Micro Electroporation by Using Microchip with a Three-dimensional Electrode Array," The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Micro TAS 2006), Tokyo, Japan, pp.28-30, 2006.
  • X. Cao and Y.-K. Lee, "Design and Fabrication of Mini Vibration Power Generator," IEEE International Conference on Information Acquisition (IEEE ICIA 2006),Weihai, China, pp.91-95, Aug 20-23, 2006.
  • M. Lee, L.S.L. Cheugn, Y.-K. Lee and Y. Zohar, "Boundary Conditions and Integrated Sensors in Microchannel Convective Heat Transfer," the 4th International Conference on Nanochannels, Microchannels and Minichannels (ASME ICNMM 2006), Limerick, Ireland, June 19-21, 2006 (No. ICNMM2006-96117) (keynote paper).
  • Y.C. Chan, Y.-K. Lee  and Y. Zohar, "Nonlinear Electrophoretic Mobility of Large DNA Molecules in Microsystem with Sub-Micron Pillar Arrays,"  IEEE MEMS 2006, Istanbul, Turkey, pp.438-441,  Jan 22-26, 2006.
  • X. Niu, Y.-K. Lee, Liyu Liu and W. Wen, "Micro valve and chaotic mixer driven by electrorheological fluid," IEEE NEMS 2006, Zhuhai, China, Jan 18-21,pp.1254-1257, 2006.
  • P.K. Leung, P. Deng, Y.-K. Lee, "Comparative Study of Size Effect of Micro Bubble Dynamics by Sub-100 Microsecond and Milisecond Pulse Heating," IEEE NEMS 2006, Zhuhai, China, Jan 18-21, pp.525-529, 2006.
  • M. Lee, Y.-K. Lee and Y. Zohar, "Two-Phase Flow in Microchannel Heat Sink with Nearly Uniform Heat Flux Boundary Condition," IEEE NEMS 2006, Zuhai, China,  Jan 18-21, pp. 754-758, 2006.
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